The interaction of histone IV with nucleoside triphosphates.
نویسندگان
چکیده
The nucleoside triphosphates ATP, GTP, TTP, and CTP induce conformational changes in histone IV at concentrations that are of the same magnitude as the histone concentration. The purine nucleoside triphosphates are much more effective than the pyrimidine nucleoside triphosphates.
منابع مشابه
Thermodynamic Studies on the Interaction of Histone H2B with Dodecyl Trimethyl Ammonium Bromide
The interaction of histone H2B and dodecyl trimethyl ammonium bromide (DTAB) was studies via equilibrium dialysis method at two different temperatures, at pH 6.4 in phosphate buffer. The binding data were used to obtain the Gibbs free energy of interaction, which is interpreted in terms of a theoretical model based on the Wyman binding potential. The data were then used to obtain...
متن کاملINTERACTION OF DNA WITH THE FOLDED AND UNFOLDED HISTONE HI IN THE PRESENCE OF SURFACE ACTIVE AGENTS
Interaction between DNA and histone H, was investigated in the presence and absence of sodium-n-dodecyl sulphate (SDS) and dodecyl trimethylammonium bromide (DTAB) at temperatures of 27 and 37?C, in 2.5 mM phosphate buffer, pH 6.4 by UV spectrophotometry, equilibriumdialysis and titration. The presence of 1.33 mM SDS caused histone H to fold and to further contact DNA. Binding data were use...
متن کاملThe effect of aspirin on the interaction of histone 05 and 05-DNA
The linker histones (H1 or H5) which play a key role in the folding of chromatin, are general repressors of gene expression. Nuclei of the mature chicken erythrocytes (and in some mammalian cells) contain both of them. Although the interaction of H5 with DNA is stronger than that of H1, it does not prevent the transcription of some erythroid-specific genes. It has been shown that some modificat...
متن کاملInitiation of synthesis of messenger RNA of deoxynucleotide kinase by oligoribonucleotides.
The effects of nucleoside triphosphates and oligoribonucleotides on the initiation of synthesis of messenger RNA of the T4 phage-specific enzyme, deoxynucleotide kinase, have been studied. The procedure involved incubation of T4 DNA, purified RNA polymerase from Escherichia coli, and selected nucleotide compounds during a brief period to permit initiation of RNA synthesis. Further initiation wa...
متن کاملHydrolysis of nucleoside triphosphates catalyzed by the recA protein of Escherichia coli. Steady state kinetic analysis of ATP hydrolysis.
The DNA-dependent ATPase activity of the recA protein of Escherichia coli shows a complex dependence on ATP concentration. With a single-stranded (SS) DNA cofactor, the Hill coefficient for ATP is 3.3 at pH 8.1 and 1.4 at pH 6.2. With a double-stranded (DS) DNA cofactor, the Hill coefficient is 3.3 at pH 6.2 (no reaction is detectable at pH 8.1). In the presence of SS DNA, the Km for ATP is 20 ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 69 9 شماره
صفحات -
تاریخ انتشار 1972